First confirmation of importation and transmission in Spain of the newly identified SARS-CoV-2 B.1.1.7 variant.

INTRODUCTION: A newly identified SARS-CoV-2 variant, VOC202012/01 originating lineage B.1.1.7, recently emerged in the United Kingdom. The rapid spread in the UK of this new variant has caused other countries to be vigilant. MATERIAL AND METHODS: We based our initial screening of B.1.1.7 on the dropout of the S gene signal in the TaqPath assay, caused by the 69/70 deletion. Subsequently, we confirmed the B.1.1.7 candidates by whole genome sequencing. RESULTS: We describe the first three imported cases of this variant from London to Madrid, subsequent post-arrival household transmission to three relatives, and the two first cases without epidemiological links to UK. One case required hospitalization. In all cases, drop-out of gene S was correctly associated to the B.1.1.7 variant, as all the corresponding sequences carried the 17 lineage-marker mutations. CONCLUSION: The first identifications of the SARS-CoV-2 B.1.1.7 variant in Spain indicate the role of independent introductions from the UK coexisting with post-arrival transmission in the community, since the early steps of this new variant in our country.

Problems of food security in the conditions of the open economy of modern Georgia

The article discusses the problems of food security in the open economy of modern Georgia against the background of the ongoing hostilities between Russia and Ukraine. After the spread of the COVID-19 pandemic, the attention of almost all countries of the world, including Georgia, was focused on self-sufficiency of the demand of their population with local agricultural and food products. In such conditions, Georgia has a chance to expand domestic production of agricultural and food products. In order to increase the competitiveness of the food and nutrition products produced in Georgia, it is necessary to improve the connections between the separate links of their production, to synchronize the production processes. In order to increase the level of food self-sufficiency of Georgia, it is necessary to protect the production of import-substituting, domestic, agrarian products with non-tariff barriers, and to properly manage their production and logistics. Significant and rapid changes in the global supply chain of food and nutrition products provoked by the Covid-19 pandemic have become an opportunity to increase the level of food self-sufficiency of Georgia, which needs to be properly used.

Ukraine's market of certified seed: current state and prospects for the future

The production of high-quality seeds and planting material is the basis for increasing the efficiency and sustainability of crop production. The main aim of this article is to develop proposals to ensure that supply meets the demand in the seed and planting material market Ukraine. The future prospects are also discussed. The paper uses statistical and comparative analysis. The patterns of foreign trade in seeds and planting material to and from Ukraine are analyzed. The high level of import dependence of Ukraine leads to excessive exposure to instability in the world seed market. The development of seed production in Ukraine is discussed and analyzed along with the ways for improving commercial circulation of seeds and planting materials. The export volumes of grains and oilseeds in 2020 were the highest of those over the last three decades and amounted to USD 18.7 million, which is almost twice more than in 2019. Corn seeds dominate in exports (72%). The volume of imports of seeds of grains and oilseeds exceeded exports by 22 times in value and, in 2020, amounted to USD 409.4 million. In the total volume of imports, imports of sunflower seeds accounted for 53%. The upward trend of seed imports has been maintained since 2010. It was the result of increased demand for imported seeds by large- and medium-sized agricultural producers. In 2020, COVID-19 gave impetus to the development of domestic seed production and foreign breeding companies within the country. Prospective ways to accelerate the development of the organization of the Ukrainian seed and planting material market are outlined. Ukraine has prospects for increasing the export of seeds of grains and oilseeds by expanding its production by foreign companies operating in Ukraine. Solving problems of competitiveness seed production in Ukraine will make it possible to strengthen the role of domestic breeding in the seed market, as well as to use the best foreign varieties through their commercial circulation.

Vector-borne and other pathogens of potential relevance disseminated by relocated cats.

Large populations of unowned cats constitute an animal welfare, ecological, societal and public health issue worldwide. Their relocation and homing are currently carried out in many parts of the world with the intention of relieving suffering and social problems, while contributing to ethical and humane population control in these cat populations. An understanding of an individual cat's lifestyle and disease status by veterinary team professionals and those working with cat charities can help to prevent severe cat stress and the spread of feline pathogens, especially vector-borne pathogens, which can be overlooked in cats. In this article, we discuss the issue of relocation and homing of unowned cats from a global perspective. We also review zoonotic and non-zoonotic infectious agents of cats and give a list of practical recommendations for veterinary team professionals dealing with homing cats. Finally, we present a consensus statement consolidated at the 15th Symposium of the Companion Vector-Borne Diseases (CVBD) World Forum in 2020, ultimately to help veterinary team professionals understand the problem and the role they have in helping to prevent and manage vector-borne and other pathogens in relocated cats.

The risk of SARS-CoV-2 Omicron variant emergence in low and middle-income countries (LMICs).

We estimated the probability of undetected emergence of the SARS-CoV-2 Omicron variant in 25 low and middle-income countries (LMICs) prior to December 5, 2021. In nine countries, the risk exceeds 50 %; in Turkey, Pakistan and the Philippines, it exceeds 99 %. Risks are generally lower in the Americas than Europe or Asia.

First confirmation of importation and transmission in Spain of the newly identified SARS-CoV-2 B.1.1.7 variant.

INTRODUCTION: A newly identified SARS-CoV-2 variant, VOC202012/01 originating lineage B.1.1.7, recently emerged in the United Kingdom. The rapid spread in the UK of this new variant has caused other countries to be vigilant. MATERIAL AND METHODS: We based our initial screening of B.1.1.7 on the dropout of the S gene signal in the TaqPath assay, caused by the 69/70 deletion. Subsequently, we confirmed the B.1.1.7 candidates by whole genome sequencing. RESULTS: We describe the first three imported cases of this variant from London to Madrid, subsequent post-arrival household transmission to three relatives, and the two first cases without epidemiological links to UK. One case required hospitalization. In all cases, drop-out of gene S was correctly associated to the B.1.1.7 variant, as all the corresponding sequences carried the 17 lineage-marker mutations. CONCLUSION: The first identifications of the SARS-CoV-2 B.1.1.7 variant in Spain indicate the role of independent introductions from the UK coexisting with post-arrival transmission in the community, since the early steps of this new variant in our country.

The analysis of the characteristics of imported COVID-19 cases from January to April in 2020: a cross-sectional study.

Background: Since the first case reported in December 2019, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an outbreak of coronavirus disease 2019 (COVID-19) worldwide. The global case count continued to rise and the WHO declared a Public Health Emergency of International Concern (PHEIC), causing a growing risk of imported COVID-19 infection. This study aimed to provide descriptive and quantitative epidemiological characteristics of imported COVID-19 cases in China. Methods: This cross-sectional study examined all imported COVID-19 cases in Mainland China from 22 January to 21 April 2020. Ratios, Median and percentile were used for descriptive analysis. Spearman's correlation analysis was performed between daily new imported cases in Mainland China and the country of origin. The chi-square test was used to evaluate the difference between home quarantine and compulsory centralized quarantine on native transmission. Results: A total of 1,610 cases of COVID-19 were imported from 49 countries to 27 provincial administrative regions in China; 79.8% were from European countries and the United States of America (the USA). Before 29 March 2020, the imported cases were mainly from the USA (27.7%) and United Kingdom (UK; 42.6%). After 29 March 2020, the daily newly imported cases from Russia rapidly grew. After 12 April 2020, the number of daily newly imported cases gradually decreased and remained at a low level (12±7 cases per day). Airport entry was encouraged, and ground border crossing was limited. Among the 1,610 cases, 54.0% were in the asymptomatic incubation period on arrival in Mainland China. Conclusions: The transmissions by imported COVID-19 were gradually and effectively curbed in Mainland China, despite a disproportionally high number of cases worldwide. Entry screening measures must be implemented universally to all inbound travelers at a point of entry or targeted to specific travel routes or to specific travelers. Compulsory centralized quarantine should be recommended in the prevention of the imported COVID-19 epidemic.

The State of Pharmaceutical Manufacturing in the Democratic Republic of Congo: The Journey so Far.

Despite a population of over 89 million people, the Democratic Republic of Congo (DRC) has just 30 local pharmaceutical manufacturers (DRC) with the majority of manufacturers based in Kinshasa, the capital city. Of the total number of pharmaceutical products sold in the DRC, just 10% accounts for those manufactured locally. The DRC remains dependent on other countries such as China and India for the supply of active pharmaceutical ingredients (APIs) -thus, local pharmaceutical manufacturing is therefore reliant on imported commodities and industrial machinery. Given the relevance of the pharmaceutical industry, it is vital to pay careful attention to the problems affecting it. Here, we assess the current situation of pharmaceutical manufacturing in the DRC, examine current challenges, and provide solutions for future development.

Aligning conservation and public health goals to tackle unsustainable trade of mammals

Unsustainable wildlife trade is a major driver of biodiversity loss and an important public health threat. Yet, effective wildlife trade regulation is currently at odds with food security and economic incentives provided by this global, multibillion-dollar industry. Given such limitations, public health and conservation resources can be aligned to target species for which trade both increases risk of extinction and threatens public health. Here, we developed a simple conservation and health trade risk (CHT) index (range: 2-50) using a case study of traded mammals based on species' extinction and zoonotic risks, weighed by the extent of their trade. We applied this index to 1161 International Union for the Conservation of Nature-listed terrestrial mammals involved in the wildlife trade to identify 284 high-priority species that scored high in the CHT index (CHT >= 18). Species ranking high for conservation, public health, and trade risks include those belonging to the orders Primates, Cetartiodactyla (even-toed ungulates), Rodentia (rodents), Chiroptera (bats), and Carnivora (carnivores). Of the high-priority species, 33% (n = 95) are country-endemics and may be good candidates for trade regulations and enforcement at national scales. Our study provides a preliminary step in prioritizing species, taxonomic groups, and countries for focused wildlife trade regulation to meet both conservation and public health goals.

Travel-related Importation and Exportation Risks of SARS-CoV-2 Omicron Variant in 367 Prefectures (Cities) - China, 2022.

Introduction: Minimizing the importation and exportation risks of coronavirus disease 2019 (COVID-19) is a primary concern for sustaining the "Dynamic COVID-zero" strategy in China. Risk estimation is essential for cities to conduct before relaxing border control measures. Methods: Informed by the daily number of passengers traveling between 367 prefectures (cities) in China, this study used a stochastic metapopulation model parameterized with COVID-19 epidemic characteristics to estimate the importation and exportation risks. Results: Under the transmission scenario (R0 =5.49), this study estimated the cumulative case incidence of Changchun City, Jilin Province as 3,233 (95% confidence interval: 1,480, 4,986) before a lockdown on March 14, 2022, which is close to the 3,168 cases reported in real life by March 16, 2022. In a total of 367 prefectures (cities), 127 (35%) had high exportation risks according to the simulation and could transmit the disease to 50% of all other regions within a period from 17 to 94 days. The average time until a new infection arrives in a location in 1 of the 367 prefectures (cities) ranged from 26 to 101 days. Conclusions: Estimating COVID-19 importation and exportation risks is necessary for preparedness, prevention, and control measures of COVID-19 - especially when new variants emerge.

Chemistry Manufacturing and Controls Development, Industry Reflections on Manufacture, and Supply of Pandemic Therapies and Vaccines.

This publication provides some industry reflections on experiences from the Chemistry, Manufacturing, and Controls (CMC) development and manufacture and supply of vaccines and therapies in response to the COVID-19 pandemic. It integrates these experiences with the outcomes from the collaborative work between industry and regulators in recent years on innovative science- and risk-based CMC strategies to the development of new, high-quality products for unmet medical needs. The challenges for rapid development are discussed and various approaches to facilitate accelerated development and global supply are collated for consideration. Relevant regulatory aspects are reviewed, including the role of Emergency Use/Conditional Marketing Authorizations, the dialogue between sponsors and agencies to facilitate early decision-making and alignment, and the value of improving reliance/collaborative assessment and increased collaboration between regulatory authorities to reduce differences in global regulatory requirements. Five areas are highlighted for particular consideration in the implementation of strategies for the quality-related aspects of accelerated development and supply: (1) the substantial need to advance reliance or collaborative assessment; (2) the need for early decision making and streamlined engagement between industry and regulatory authorities on CMC matters; (3) the need to further facilitate 'post-approval' changes; (4) fully exploiting prior and platform knowledge; and (5) review and potential revision of legal frameworks. The recommendations in this publication are intended to contribute to the discussion on approaches that can result in earlier and greater access to high-quality pandemic vaccines and therapies for patients worldwide but could also be useful in general for innovative medicines addressing unmet medical needs.

First importations of SARS-CoV-2 P.1 and P.2 variants from Brazil to Spain and early community transmission.

INTRODUCTION: SARS-CoV-2variants of concern (VOC) have been described in the UK (B.1.1.7), South Africa (B.1.351) and Brazil (P.1). Among them, the most scarce information has been obtained from the P.1 variant and more data on its global presence and about its spreading dynamics are needed. METHODS: Whole genome sequencing was performed prospectively on travellers arriving from Brazil and on a random selection of SARS-CoV-2 positive cases from our population. RESULTS: In this study we report the first SARS-CoV-2 P.1 and P.2 variants exported from Brazil to Spain. The case infected with the P.1 variant, who had only stayed in Rio de Janeiro, required hospitalisation. The two P.2 cases remained asymptomatic. A wider distribution for P.1 variant beyond the Brazilian Amazonia should be considered. The exportation of the P.2 variant, carrying the E484K mutation, deserves attention. One month after the first description of P.1 and P.2 importations from Brazil to Madrid, these variants were identified circulating in the community, in cases without a travel history, and involved in household transmissions CONCLUSION: Whole genome sequencing of SARS-CoV-2 positive travellers arriving from Brazil allowed us to identify the first importations of P.1 and P.2 variants to Spain and their early community transmission.

Importation, Local Transmission, and Model Selection in Estimating the Transmissibility of COVID-19: The Outbreak in Shaanxi Province of China as a Case Study.

Background: Since the emergence of the COVID-19 pandemic, many models have been applied to understand its epidemiological characteristics. However, the ways in which outbreak data were used in some models are problematic, for example, importation was mixed up with local transmission. Methods: In this study, five models were proposed for the early Shaanxi outbreak in China. We demonstrated how to select a reasonable model and correctly use the outbreak data. Bayesian inference was used to obtain parameter estimates. Results: Model comparison showed that the renewal equation model generates the best model fitting and the Susceptible-Exposed-Diseased-Asymptomatic-Recovered (SEDAR) model is the worst; the performance of the SEEDAR model, which divides the exposure into two stages and includes the pre-symptomatic transmission, and SEEDDAAR model, which further divides infectious classes into two equally, lies in between. The Richards growth model is invalidated by its continuously increasing prediction. By separating continuous importation from local transmission, the basic reproduction number of COVID-19 in Shaanxi province ranges from 0.45 to 0.61, well below the unit, implying that timely interventions greatly limited contact between people and effectively contained the spread of COVID-19 in Shaanxi. Conclusions: The renewal equation model provides the best modelling; mixing continuous importation with local transmission significantly increases the estimate of transmissibility.

SARS-CoV-2 surveillance in imported meat and animal parts for food safety and security

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent for the coronavirus disease 2019 (COVID-19) pandemic. COVID-19 is contagious and fatal to humans. In the context of the COVID-19 pandemic, significant concerns on food safety and security are rising due to potential interspecies transmission. As such, surveillance of SARS-CoV-2 on imported meat and animal parts is carried out and reported in this study to safeguard food safety and security. Overall, none of the 225 samples from various livestock (buffaloes, cattle, goat and pig) imported from seven countries were tested positive for SARS-CoV-2 with quantitative reverse transcription polymerase chain reaction (RT-qPCR) from July 2020 to November 2021. This study finding serves as a baseline data for SARS-CoV-2 in imported meat and animal parts. Notably, this study accentuated the importance of active surveillance to prevent zoonosis and to safeguard food safety and security.

Mathematical Modeling for Removing Border Entry and Quarantine Requirements for COVID-19, Vanuatu.

The Pacific Island country of Vanuatu is considering strategies to remove border restrictions implemented during 2020 to prevent imported coronavirus disease. We performed mathematical modeling to estimate the number of infectious travelers who had different entry scenarios and testing strategies. Travel bubbles and testing on entry have the greatest importation risk reduction.

Telling the Wood from the Trees: Ranking a Tree Species List to Aid Urban Afforestation in the Amazon

The vast Amazonian biome still poses challenges for botanists seeking to know and recognize its plant diversity. Brazilian northern cities are expanding fast, without considering the regional biodiversity, and urban plantings of almost exclusively exotic species are taking place. It is paramount that the correct identity of such trees is ascertained before procurement of the seeds and young plants, as the use of popular names may lead to importation of plant material from elsewhere, with potential introduction of invasive species. The abundant local diversity also leads to the need to score the most suitable species within a given region. Following the preparation of authoritatively named floristic lists in Southeastern Pará state, we proceeded to score and rank the most suitable trees for urban planning using different characteristics such as size, ornamental value, ecologic role, resilience and known methods of propagation. From an initial 375 species list, 263 species were ranked according to their suitability for street and urban area plantings and visualized using a Venn diagram. A final list with the 49 of the highest-ranking species was further analysed regarding their pollination and phenology period and two types of dissimilarity analyses were provided to aid practitioners in matching and choosing groups of species. Different local vegetation types mean that similar floristic lists must be used to extract cohorts of suitable plants to increase the urban richness in the eight Brazilian states that are included in the Amazonian biome.

RiskEstim: A Software Package to Quantify COVID-19 Importation Risk

We present an R package developed to quantify coronavirus disease 2019 (COVID-19) importation risk. Quantifying and visualizing the importation risk of COVID-19 from inbound travelers is urgent and imperative to trigger public health responses, especially in the early stages of the COVID-19 pandemic and emergence of new SARS-CoV-2 variants. We provide a general modeling framework to estimate COVID-19 importation risk using estimated pre-symptomatic prevalence of infection and air traffic data from the multi-origin places. We use Hong Kong as a case study to illustrate how our modeling framework can estimate the COVID-19 importation risk into Hong Kong from cities in Mainland China in real time. This R package can be used as a complementary component of the pandemic surveillance system to monitor spread in the next pandemic.

L’agriculture burkinabè face à la crise de la Covid-19 : cas des régions du Yatenga et des Hauts-Bassins

Au début de la crise sanitaire due à la Covid-19, certains observateurs ont alerté les décideurs d’une possible crise alimentaire dans les mois à venir en Afrique de l’Ouest. En vue d’alimenter ce débat, nous avons mené une étude en nous entretenant avec 75 acteurs du secteur agricole dans deux régions du Burkina Faso − le Yatenga et les Hauts-Bassins. Dans les deux situations, les agriculteurs et les éleveurs ont pu continuer leurs activités mais certains ont eu des difficultés de commercialisation. Les maraîchers et les arboriculteurs ont été les plus touchés par cette crise du fait des difficultés à écouler leurs produits périssables sur les marchés ouest-africains. Les éleveurs ont été moins concernés par la baisse des prix mais les commerçants de bétail sur pied ont aussi rencontré des difficultés à exporter vers les pays côtiers. Enfin, les sociétés cotonnières ont dû faire face à une baisse du prix international du coton-fibre et les producteurs de coton ont dû faire face à une baisse du prix d’achat du coton-graine à la fin de 2020. Malgré cette crise, l’agriculture burkinabè a continué à jouer pleinement son rôle nourricier grâce à la mobilisation des agriculteurs, des commerçants et des transporteurs, même si elle montre des fragilités dues à sa forte dépendance aux marchés extérieurs pour entre autres les légumes, le bétail, le coton, les mangues, l’anacarde et les intrants agricoles et d’élevage. Cette crise permet de réfléchir à des axes d’intervention afin de rendre l’agriculture burkinabè moins dépendante des marchés extérieurs et des facteurs de production importés. Cela implique la substitution des produits alimentaires importés par des produits locaux et une transition agroécologique permettant de réduire l’importation d’intrants de synthèse.Alternate : At the beginning of the health crisis due to COVID-19 some observers alerted the decision makers of a possible food crisis in the coming months in West Africa. In order to feed this debate, we conducted a study by interviewing 75 actors of the agricultural sector in two regions of Burkina Faso − the Yatenga and the High Basins. In both situations, farmers and pastoralists were able to continue their activities, but some experienced marketing difficulties. Market gardeners and tree growers have been the most affected by this crisis because of difficulties in selling their perishable products on West African markets. Livestock farmers were less affected by the drop in prices, but traders of live livestock also encountered difficulties exporting to coastal countries. Finally, cotton companies have had to face a drop in the international price of cotton fiber and cotton producers will have to face a drop in the purchase price of seed cotton at the end of 2020. Despite this crisis, Burkina Faso’s agriculture has continued to fully play its nurturing role thanks to the mobilization of farmers, traders and transporters, even though it is showing weaknesses due to its heavy dependence on external markets for, among other things, vegetables, livestock, cotton, mangoes, cashew nuts and agricultural and livestock inputs. This crisis is an opportunity to consider areas for intervention to make Burkina Faso’s agriculture less dependent on external markets and imported factors of production. This implies the substitution of imported food products by local products and an agro-ecological transition to reduce the importation of synthetic inputs.

INTELLIGENT LOW-COST PRELIMINARY IDENTIFICATION OF COVID-19 USING IOT

: The World Health Organization(WHO)has proclaimed a worldwide health emergency of international concern due to the coronavirus (COVID-19)disease outbreak. This viral outbreak has caused more than 2,863,225 deaths in the world. It has spread over into all areas of the globe. Excessive national and international action is being taken to stop the outbreak. The WHO suggested taking the necessary steps and measures to reduce the risk of the disease or importation.WHO's suggested measures are not to contact the infected person and do not touch the frequently used areas. People are observing these suggestions, but it is still spreading. The process of vaccination around the world has started. Coronavirus disease can be avoided or stopped, with the instant widespread of internet technologies. Current Internet of Things (IoT) developments on coronary virus protection is discussed in this paper from a fever control point of view on airports, religious sites, borders, events, etc. The design of the technique developed in this paper is a very low-cost remote temperature monitoring system model IoT Naïve Bayesian (INB) which measures body temperature by the sensor with infrared rays, processes and learns intelligently with Naïve Bayesian System and sends the data to a cloud system without any human intervention. It is extremely useful in preventing the epidemic on airports, religious sites, border crossings, and activities, among other places.

The impact of vaccination status on importation of COVID-19 among international travellers.

Governments worldwide are looking for ways to safely enable international travel while mitigating the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and the associated coronavirus disease 2019 (COVID-19). However, few data describe the impact of vaccination on importation of COVID-19. We took advantage of the sequential introduction of two government policies in Canada to evaluate the real-world evidence of vaccine effectiveness among 30,361 international travellers arriving by air in Alberta, Canada. The proportion of COVID-19-positive results for travellers who were either fully vaccinated or partially vaccinated was 0.02% (95% CI: 0.00-0.10) (i.e. one positive case among 5,817 travellers). In contrast, 1.42% (95% CI: 1.27-1.58) of unvaccinated travellers tested positive for SARS-CoV-2 (341 cases among 24,034 travellers). These findings suggest that COVID-19 vaccinations approved in Canada, substantially reduced the risk of travel-related importation of COVID-19 when combined with other public health measures. The low absolute rate of infection among fully vaccinated or partially vaccinated international travellers may inform quarantine requirements in this population.